please enter your summer class schedule here (if you are also taking classes in the summer or have other conflicts with lab work): (Link )
Mentor Schedules
Want to know when the mentors will be on? - check here __(Link)__
Start Date: Thursday June 2nd 2016 -----------------------------------> End Date: ~ end of July sometime 2016
Group Meeting/Journal Club
once a week Location: GEA 100 ????? See Journal Club link on left hand side (lhs) menu Group Meeting Slides are in the Google Drive folder
Spaces
PAI 2.14 wet lab GEA - the fancy Human Ecology building PAI 3.04H Dr. B's office WEL 2.128 computer lab, WEL 2.144 computer lab Painter 1st floor - ice room, autoclave, big centrifuge Biotech lab - WEL 3.270 Robertus lab - WEL 5th floor Chemistry Storeroom - WEL 1st floor (basement) - supplies and dry ice Chemistry Electronics Shop - WEL 2nd floor (near computer lab) BioScience Storeroom - NHB 1.502 - supplies
ICMB Storeroom - MBB 1.426K (separate room for turning in invoice and for picking up items)
Can only pick up items from 9-12 AM and 2-5 PM
The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise. First, we will review those and see if they are good. Order overlap primers for our targets (we need these before we can start cloning for 'real') While we wait on the primers, we will express, purify and characterize YopH & FtHap enzymes - 2 current VDS targets. -- may also do NDM-1 and RpFabG
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills. Then we will proceed to work on cloning our targets Then we will express, purify and characterize our targets. Then we will perform enzyme activity assays to see if our targets work Then we will carry out inhibition assays
Wikispaces no like it when you have more than 3 windows open at a time! It be slow. Store reagents at the proper temperature & in the right location (e.g. correct freezer box) Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours) Write down everything that you do in lab into your lab notebook Clean your area when done and return reagents and materials to their 'homes'
What are we doing today?
see the Daily Schedule Summ16 (editable)==> Edit your Summer Student Schedules
Week 0: 2-Jun Thursday 5-Jun Sunday
Week 1: 6-Jun Monday 12-JunSunday
Week 2: 13-JunMonday 19-JunSunday
Week 3: 20-JunMonday 26-JunSunday
Week 4: 27-JunMonday 3-Jul Sunday
Week 5: 4-Jul Monday 10-Jul Sunday
Week 6: 11-Jul Monday 17-Jul Sunday
Week 7: 18-Jul Monday 24-Jul Sunday (change?)
Week 8: 25-Jul Monday 29-Jul Friday
Summer Researchers:
Mentors: Ana, Steven, Krupa, ARI Mentors: Andee F, Charina B,Old School Mentors: Melissa, Grace
Super Researcher: Steven
ROSTER:
VIEW
https://docs.google.com/spreadsheets/d/1qPFVFrMbxfsPZck4FYEL1YNrDcGUZInv521vnCh7MN4/pubhtml
EDIT
https://docs.google.com/spreadsheets/d/1qPFVFrMbxfsPZck4FYEL1YNrDcGUZInv521vnCh7MN4/edit#gid=0
Schedules
please enter your summer class schedule here (if you are also taking classes in the summer or have other conflicts with lab work):(Link )
Mentor Schedules
Want to know when the mentors will be on? - check here __(Link)__Start Date: Thursday June 2nd 2016 -----------------------------------> End Date: ~ end of July sometime 2016
Group Meeting/Journal Club
once a weekLocation: GEA 100 ?????
See Journal Club link on left hand side (lhs) menu
Group Meeting Slides are in the Google Drive folder
Spaces
PAI 2.14 wet labGEA - the fancy Human Ecology building
PAI 3.04H Dr. B's office
WEL 2.128 computer lab, WEL 2.144 computer lab
Painter 1st floor - ice room, autoclave, big centrifuge
Biotech lab - WEL 3.270
Robertus lab - WEL 5th floor
Chemistry Storeroom - WEL 1st floor (basement) - supplies and dry ice
Chemistry Electronics Shop - WEL 2nd floor (near computer lab)
BioScience Storeroom - NHB 1.502 - supplies
- NHB glassware shop - next to BioSci storeroo
ICMB Core Facilities:The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise.First, we will review those and see if they are good.
Order overlap primers for our targets (we need these before we can start cloning for 'real')
While we wait on the primers, we will express, purify and characterize YopH & FtHap enzymes - 2 current VDS targets.
-- may also do NDM-1 and RpFabG
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills.
Then we will proceed to work on cloning our targets
Then we will express, purify and characterize our targets.
Then we will perform enzyme activity assays to see if our targets work
Then we will carry out inhibition assays
Lab Safety:
Have you complete your lab safety trainings?Tours and Fun Stuff
TACC Tour at VisLab in ACES building ON CAMPUS - @
TACC Machine room Tour - @
Field Trip Form - Summer 2016
GASF (Genome Sequencing and Analysis Facility) Tour in MBB 4.102 (not done)
Skills List:
Target DiscoveryPCR (primer overlap)
Restriction Enzyme Digest
Cloning
Plasmid Midi-Prep
DNA Sequencing
Protein Expression, Purification, Characterization (Large scale)
FPLC
Enzyme assays
Things to Keep in Mind
Wikispaces no like it when you have more than 3 windows open at a time! It be slow.Store reagents at the proper temperature & in the right location (e.g. correct freezer box)
Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours)
Write down everything that you do in lab into your lab notebook
Clean your area when done and return reagents and materials to their 'homes'